Autologous hematopoietic stem cell transplantation in 32 patients with aggressive relapsing-remitting multiple sclerosis

Jun 17, 2023 | Neurosciences

A new nationwide retrospective single center study by Danish authors reported the results of autologous hematopoietic stem cell transplantation (AHSCT) in 32 patients with relapsing-remitting multiple sclerosis (MS).

Multiple sclerosis (MS) is a chronic, immune-mediated, demyelinating disease of the central nervous system that is caused by a multifactorial interaction of genetic and environmental factors. MS is characterized by multiple areas of inflammation and demyelination in the white matter of the brain and spinal cord. The clinician manifestations are protean, being determined by the various locations and extent of the demyelinating foci. Central nervous system demyelinating lesions, MS plaques, are characterized by infiltration of mononuclear cells, the proliferation of macrophages, and loss of oligodendrocytes, myelin-producing cells. 

The majority of patients diagnosed with relapsing-remitting MS are successfully treated with disease-modifying therapies. However, some patients may develop a more aggressive course of relapsing-remitting MS which is refractory to such therapies. Since 1995, MS patients have been treated with autologous hematopoietic stem cell transplantation (AHSCT) with the aim of eliminating autoreactive immune cells with high-dose chemotherapy, followed by immune reconstitution after AHSCT. A retrospective evaluation of AHSCT demonstrated a poor response in patients with chronic progressive MS and a better response in younger patients with aggressive relapsing disease.

Two conditioning protocols are commonly used for AHSCT, the myeloablative BEAM regimen with carmustine, etoposide, cytarabine arabinoside, melphalan and antithymocyteglobulin (ATG), and the non-myeloablative CY/ATG regimen with cyclophosphamide (CY) and ATG. According to the European Group for Blood and Marrow Transplantation (EMBT) 2020 guidelines, there is no evidence suggesting a difference in efficacy or safety between the two types of regimens. Therefore, both types of AHSCT regimens are now recommended for patients with MS.

About the study

The present study is a retrospective observational study of all Danish patients treated with AHSCT at a single institution. The inclusion criteria for treatment were: (1) diagnosis of aggressive relapsing-remitting MS, defined as either (a) severe disabling relapses and extensive magnetic resonance imaging (MRI) activity in untreated patients, (b) at least two severe, documented relapses and typical MRI activity in patients treated with first-line treatment, or (c) one documented severe relapse and typical MRI activity in patients treated with second-line treatment; (2) age 18–50 years, and (3) an Expanded Disability Status Scale (EDSS) score between 3.0-6.0.

For treatment were not eligible patients with progressive MS, Eastern Cooperative Oncology Group performance status score >2, pulmonary function test with forced expired volume in the first second or diffusion capacity below 40% of expected, and heart failure with left ventricular ejection fraction under 45%.

Patients underwent both hematological and neurological follow-up for the first 3-months after AHSCT and thereafter at 6-months, 12-months, 18-months, and 24-months. The adverse events (AEs) included toxicities from the chemotherapy, side effects from prophylactic treatment, infections, and other events during admission that patient had never had before.

From May 2011 to May 2021, 32 patients underwent autologous hematopoietic stem cell transplantation. Seven patients underwent AHSCT with carmustine, etoposide, cytarabine arabinoside, melphalan and ATG regimen (BEAM/ATG), with a median follow-up of 49 months. Twenty-five patients underwent AHSCT with cyclophosphamide and ATG regimen (CY/ATG), with a median follow-up of 39 months. The two groups of patients had significant differences in regard to age, disease duration, baseline EDSS and relapse rate one year before AHSCT.

Across the whole cohort, relapse-free survival was 77%, worsening-free survival was 79%, MRI event-free survival was 93%, and no evidence of disease (NEDA-3) was 69% at the end of the second year after the AHSCT. There was no treatment related mortality. The CY group had significantly higher two-year relapse-free survival (83%) compared to the BEAM group (57%), and significantly higher two-year worsening-free survival (85%) compared to the BEAM group (57%). The observed significant differences in relapse-free survival, worsening-free survival, and NEDA-3 between groups became insignificant after adjusting for sex, age, disease duration, baseline EDSS and relapse rate one-year before AHSCT.

During conditioning and the days after admission, the most common AEs reported were nausea and vomiting, diarrhea, dyspepsia or reflux, abdominal pain, obstipation, mucositis, thrombocytopenia, anemia, neutropenic fever, infections, fatigue, fever, headache, and neurogenic bladder. After discharge, the most common AEs were various infections that required hospitalization. Other relevant AEs after discharge included thyroid diseases and neoplasia. Less than three 3 BEAM patients (14%) and seven CY patients (28%) developed thyroid diseases post-AHSCT. Less than three BEAM patients (14%) developed cervical dysplasia five years after AHSCT, and less than three 3 CY patients (5%) developed a prolactinoma or a meningioma two years after AHSCT. There was no evidence of treatment-related mortality. One patient who was treated died six years later.

In conclusion, these findings demonstrated that AHSCT was a relatively safe and effective treatment for Danish patients with aggressive relapsing-remitting MS. There were few serious AEs and no deaths. The less aggressive non-myeloablative CY/ATG regimen was as effective as myeloablative BEAM/ATG regimen, with fewer adverse events.

This article was published in Multiple Sclerosis and Related Disorders.

Journal Reference

Jespersen F et al. Autologous hematopoietic stem cell transplantation of patients with aggressive relapsing-remitting multiple sclerosis: Danish Nation-wide experience, Multiple Sclerosis and Related Disorders (2023).